Amphyllis Twigden, daughter of John Twigden and Anne Dickens, was baptized on February 2, 1602 in Spratton, Northamptonshire, England and was buried on January 19, 1655 in Tring, Hertfordshire, England at the age of 52. She married Lawrence Washington, son of Lawrence Washington and Margaret Butler, in December 1633 in Purleigh, Essex, England. He was born in February 1601 in Sulgrave Manor, Northamptonshire, Englan and died in January 1653 at the age of 51.

John Washington, colonel, son of Lawrence Washington and Amphyllis Twigden, was born in 1633 in Purleigh, Essex, England and died in 1676 in Washington Paris, Westmoreland Co., VA at the age of 43. He married Anne Pope, daughter of Nathaniel Pope and Lucy …, on December 1, 1658 in Westmoreland Co., VA. She died in 1667 in Westmoreland Co., VA.

Lawrence Washington, captain, son of John Washington and Anne Pope, was born in September 1659 in Westmoreland Co., Virginia and died in February 1698 in Warner Hall, Gloucester Co., Virginia at the age of 38. He married Mildred Warner, daughter of Augustine Warner and Mildred Reade, in 1688. She was born in 1670 in Warner Hall, Gloucester Co., Virginia and died on March 26, 1701 in Whitehaven, Cumberland, England at the age of 31.